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T6146

Trypan Blue

powder, BioReagent, suitable for cell culture

Sinónimos:

Direct blue 14, Niagara blue reagent, blue live/dead cell assay

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Fórmula lineal:
C34H24N6O14S4Na4
Número CAS:
72-57-1
Peso molecular:
960.81
EC Number:
200-786-7
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
57654686
MDL number:
MFCD00003969
Beilstein/REAXYS Number:
4360496
Colour Index Number:
23850
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product line

BioReagent

Quality Level

300

form

powder

composition

Dye content, ~40%

packaging

glass bottle of 100 g, glass bottle of 25 g, glass bottle of 5 g

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

mp

>300 °C (lit.)

solubility

H2O: 10 mg/mL

application(s)

cell analysis

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770), PTPRF(5792)

General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:


  • in cell viability assay
  • to stain plant tissue in order to to visualize cell death

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Preparation Note

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificados de análisis (COA)

Lot/Batch Number
23796BJ
25496TH
09614EH

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Plant tissue trypan blue staining during phytopathogen infection.
Fernandez NB, et al.
Bio-protocol (2016)
A histological analysis of lens capsules stained with trypan blue for capsulorrhexis in phacoemulsification cataract surgery
Singh AJ, et al.
Eye (London, England), 17(5), 567-567 (2003)
Trypan blue exclusion test of cell viability
Strober W
Current Protocols in Immunology, 21(1), A-3B (1997)
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