{"lastModifiedDate":1622794961722,"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"publishedDate":1668829434954,"lastModifiedDate":1622794961722,"keywords":["Protocol","Large molecule HPLC"],"title":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution","seoSchemas":[{"itemListElement":[{"position":1,"name":"Large Molecule HPLC","item":"%%HOMEPAGE_URL%%/INTL/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc","@type":"ListItem"},{"position":2,"name":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution","@type":"ListItem"}],"@context":"https://schema.org","@type":"BreadcrumbList"}],"description":"Protocol for HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution","pageType":"Protocol","ogImageMetaInfo":[{"ogTitle":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution","ogType":"website","ogImage":"https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/social-media/vibrant-m/vibrant-m.jpg","ogUrl":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119","ogDescription":"Protocol for HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution"}],"translationMethod":null,"canonicalUrl":null},"designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","title":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution","templateName":"technical-article---protocol-page-template","language":"en",":itemsOrder":["root"],":items":{"root":{"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columnCount":12,"allowedComponents":{"applicable":false,"components":[{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/genericPlaceholder","title":"Generic Placeholder"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/productRecommendationCarousel","title":"Product Recommendation Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/productselector","title":"Product Selector Tool"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/recommendedSearch","title":"Recommended Search Term"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/selfServiceReviewForm","title":"Self Service Review Form"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/shopProductRightRail","title":"Shop Product Right Rail"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/sitemap","title":"Site Map"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/tableofcontents","title":"Table of Contents"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/textbanner","title":"Text Banner"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/topPagesCarousel","title":"Top Pages Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/userEngageCarousel","title":"User Engage Carousel"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-175d6c5542","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":["tagbasedbreadcrumb","title"],":items":{"tagbasedbreadcrumb":{"items":[{"url":"/","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc","title":"Large Molecule HPLC","titleEng":"Large Molecule HPLC","active":false,"nonClickable":false},{"url":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119","title":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution","titleEng":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"openNewTab":false,"linkURL":null,"text":"HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution","type":"h1",":type":"cms-commons/components/content/title"}},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-f71427af25","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":[],":items":{},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-f8889866ca","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--8 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","table_814752941":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-44969ca3e6","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":["image","table","table_814752941"],":items":{"image":{"anchorId":null,"src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/analytical-chemistry/large-molecule-hplc/bovine-serum-albumin/bovine-serum-albumin.jpg","externalUrl":false,"title":"bovine-serum-albumin","uuid":"5a98a308-28bd-4c15-9b4f-7137469a1398","alt":"bovine-serum-albumin","link":"","widths":[],"caption":"","displayPopupTitle":false,":type":"cms-commons/components/content/image"},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

column	SRT SEC-300, 30 cm x 7.8 mm I.D., 5 µM, 300 Angstrom particles (Z777051)
column	Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µM, 300 Angstrom particles (Z777033)
column temp.	ambient
mobile phase	150 mM sodium phosphate, pH 7
flow rate	1 mL/min
sample	5 mg/mL, BSA
injection	10 µL
detector	UV, 214 nm

\r\n",":type":"cms-commons/components/content/table"},"table_814752941":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Categories	Analytical Chromatography, Clinical, Proteins, proteins
Featured Industry	Life Science and Biopharma \r\nPharmaceutical (small molecule)
Legal Information	SRT is a registered trademark of Sepax Technologies, Zenix is a registered trademark of Sepax Technologies
suitability	application for HPLC

\r\n",":type":"cms-commons/components/content/table"}},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"dynamicproducttable":{"anchorId":null,"productsArray":["5.33001 Supelco (MM)","1.00029 Supelco (MM)","73594 Supelco (SIAL)","70221 Supelco (SIAL)","1.11727 Supelco (MM)","5.33002 Supelco (MM)","1.06007 Supelco (MM)","1.06035 Supelco (MM)","5.43841 Supelco (MM)","342483 Sigma-Aldrich (ALDRICH)","Z777051 Supelco (SUPELCO)","69372 Millipore (SIAL)","1.15333 Supelco (MM)","Z777033 Supelco (SUPELCO)"],"isShowImage":false,"maxRows":20,"products":[{"productKey":"533001","brandKey":"MM"},{"productKey":"100029","brandKey":"MM"},{"productKey":"73594","brandKey":"SIAL"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"111727","brandKey":"MM"},{"productKey":"533002","brandKey":"MM"},{"productKey":"106007","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"543841","brandKey":"MM"},{"productKey":"342483","brandKey":"ALDRICH"},{"productKey":"Z777051","brandKey":"SUPELCO"},{"productKey":"69372","brandKey":"SIAL"},{"productKey":"115333","brandKey":"MM"},{"productKey":"Z777033","brandKey":"SUPELCO"}],"tableTitle":"Materials",":type":"cms-commons/components/content/dynamicproducttable"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-9df52cf833","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":[],":items":{},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-e64fb83f9e","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":[],":items":{},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"}},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3","relatedcontentlinks_1464358051":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-f2287e4aee","allowedComponents":{"applicable":false,"components":[]},"unauthenticatedUserBGColor":"",":itemsOrder":["relatedcontentlinks","relatedcontentlinks_1464358051"],":items":{"relatedcontentlinks":{"contentType":"sigma-aldrich:content-type/page-type/technical-article","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/search/facet-search?focus=sitecontent&term=facet-search&facet=facet_content_type%3ATechnical+Article&facet=facet_applications%3ALarge+molecule+HPLC","title":"Related Articles","tags":["sigma-aldrich:applications/analytical-chemistry/large-molecule-hplc"],"ctaTitle":null,"categories":[{"categoryTitle":"A validated amino acid analysis assay for accurate quantification of stable isotope labeled protein reference materials: SIL thyroglobulin case study","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/amino-acid-analysis-assay-for-stable-isotope-labeled-thyroglobulin","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"New Certified Reference Materials for α‑Lactalbumin, β‑Lactoglobulin A & B, and Lactoferrin","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/new-certified-reference-materials-milk-proteins","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/filtration/quantitation-of-infliximab-and-adalimumab-by-lc-ms-ms","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"SupraSolv® GC Solvent Specifications","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/gas-chromatography/gas-chromatography-solvents","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"2 Micron SEC Column with Dual Functionality for Use in UHPLC and HPLC Systems","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/large-molecule-hplc/2-micron-size-exclusion-chromatography-column","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"A Quantitative Mass Spectrometry-based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/large-molecule-hplc/a-quantitative-mass","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Analysis of Oligonucleotides by SEC-MALS","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/large-molecule-hplc/analysis-of-oligonucleotides-by-sec-mals","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Biopolymer Separations","categoryUrl":"/technical-documents/technical-article/analytical-chemistry/large-molecule-hplc/biopolymer-separations","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"},"relatedcontentlinks_1464358051":{"linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"","title":"Related Product Categories","tags":null,"ctaTitle":null,"categories":[{"categoryTitle":"HPLC Columns","categoryUrl":"/products/analytical-chemistry/analytical-chromatography/hplc-columns","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Salts","categoryUrl":"/products/chemistry-and-biochemicals/lab-chemicals/salts","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}},"mobileSpacing":{},"desktopSpacing":{},"authenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"footer":{"id":"footer-43bd72ecb0","richText":false,":type":"cms-commons/components/content/footer"}},":type":"wcm/foundation/components/responsivegrid"}},":hierarchyType":"page",":type":"cms-commons/components/structure/page",":path":"/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-bovine-serum-albumin-bsa-g006119"}