ligand
diethylaminoethyl
description
weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
sterility
sterile (Caustic Stable)
product line
Fractogel®
form
resin
parameter
170 cm/hr flow rate
8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
100 mg binding capacity (BSA/mg of resin)
transition temp
flash point 35 °C (calculated)
density
1.430 g/cm3
bulk density
1000 kg/m3
application(s)
gene therapy
mAb
vaccine development
separation technique
weak anion exchange
storage temp.
2-30°C
Quality Level
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Analysis Note
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
Features and Benefits
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
General description
Packaging
- 1.16883.0100: Fractogel® EMD DEAE (M) Resin 100ml
- 1.16883.0010: Fractogel® EMD DEAE (M) Resin 10ml
- 1.16883.0500: Fractogel® EMD DEAE (M) Resin 500ml
- 1.16883.5000: Fractogel® EMD DEAE (M) Resin 5L
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
저장 등급
3 - Flammable liquids
wgk
WGK 3
flash_point_f
95.0 °F
flash_point_c
35 °C
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
See case study examples of how to optimize chromatographic purification of plasmid DNA for Biopharmaceutical Applications.
Free eBook outlines plasmid DNA manufacturing process and downstream purification challenges for various applications.
Learn more one the attenuated viral vaccines manufacturing process: cell culture, clarification, nuclease treatment, chromatography, and sterile filtration.
A custom-designed cost model is used to explore the economics of vaccine manufacturing across several different modalities including mRNA. The model enables greater process understanding, simulates bottlenecks, and helps to optimize production efficiency.
관련 콘텐츠
This technical article breaks down the adenovirus vaccine manufacturing process and provides a case study on developing an accelerated and cost-effective single-use adenoviral vector vaccine.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 1.16883.0100 | 04022536741949 |
| 1.16883.0010 | 04022536168708 |
| 1.16883.5000 | 04022536168722 |
| 1.16883.0500 | 04022536168715 |
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