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Merck

48-684MAG

MILLIPLEX® Ras-Raf Oncoprotein Signaling Panel

Premixed Human Ras-Raf Oncoprotein 6-Plex Cell Signaling Panel

Synonym(s):

Cell Signaling Assay, MAPK cascade assay, MEK1/ERK1/2 panel, Raf kinase assay, Ras pathway ELISA, Ras-Raf assay, Ras-Raf phosphoprotein panel, oncogene signaling multiplex, phospho-Raf

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503

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species reactivity

human

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

input

cell lysate

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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Features and Benefits

  • Simultaneously detect 6 phosphorylated proteins in the Ras-Raf-MEK-ERK signaling cascade
  • Includes c-Raf, MEK1, ERK1/2, and p90RSK
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from cancer, proliferation, and signal transduction studies
  • Ideal for oncology, targeted therapy research, and MAPK pathway analysis
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures"

General description

This panel targets phosphorylated proteins in the Ras-Raf signaling cascade, a key pathway in cell proliferation and oncogenesis. It includes c-Raf (pS338), MEK1 (pS217/pS221), ERK1/2 (pT185/pY187), and downstream effectors such as p90RSK (pT359/pS363). Dysregulation of this pathway is commonly associated with various cancers, making this panel ideal for oncology and signal transduction research.

Legal Information

Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in cell lysate samples.

Analytes Included: c-Raf (Phospho-Ser338), MEK1 (Phospho-Ser217/221), ERK1/2 (Phospho-Thr185/Tyr187), p90RSK (Phospho-Thr359/Ser363)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

Disclaimer

For research use only. Not for use indiagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which containsfluorescently labeled microsphere beads authorized by Luminex Corporation) orusing this Assay Product in any manner, you are consenting and agreeing to bebound by the End User Terms and Conditions and the End User License Agreementavailable at http://support.diasorin.com/end-user-terms-and-conditions/. If youdo not agree to all of the terms and conditions, you must promptly return thisAssay Product for a full refund prior to using it in any manner.

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Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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prtr

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Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
48-684MAG04061841049098

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