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Merck

MHSTCMAG-70K

MILLIPLEX® Mouse High Sensitivity T Cell Panel

Configurable Mouse High Sensitivity T Cell 18-Plex Panel

Synonym(s):

Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Key Documents

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Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

packaging

1 ea of

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)
standard curve range: 0.49-2,000 pg/mL
(IFNγ)
standard curve range: 0.49-2,000 pg/mL
(IL-17A)
standard curve range: 0.49-2,000 pg/mL
(IL-7)
standard curve range: 0.49-2,000 pg/mL
(TNFα)
standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)
standard curve range: 0.98-4,000 pg/mL
(IL-2)
standard curve range: 0.98-4,000 pg/mL
(IL-5)
standard curve range: 0.98-4,000 pg/mL
(IL-6)
standard curve range: 1.34-5,500 pg/mL
(IL-10)
standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))
standard curve range: 3.17-13,000 pg/mL
(LIX)
standard curve range: 3.42-14.000 pg/mL
(IL-13)
standard curve range: 3.91-16,000 pg/mL
(IL-1α)
standard curve range: 3.91-16,000 pg/mL
(IL-1β)
standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)
standard curve range: 6.1-25,000 pg/mL
(GM-CSF)
standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

cell culture supernatant(s)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Mouse T cell cytokines are critical signaling molecules produced by T cells that play essential roles in the immune response. They facilitate communication between immune cells, influencing the activation, proliferation, and differentiation of various immune cell types. Key cytokines include interleukins (such as IL-2 and IL-4) and interferons, which help orchestrate both innate and adaptive immune responses in mice.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 18 analytes in mouse serum, plasma, and cell culture supernatants.

Analytes included: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

High Sensitivity Detection: Detects picogram-level cytokine concentrations, ideal for studying subtle immune responses in low-inflammation models.

Broad Analyte Coverage: Measures 18 key cytokines including IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IFNγ, TNFα, GM-CSF, MCP-1, MIP-2, KC, LIX, and IL-7.

Verified for In Vitro and In Vivo Models: Proven performance in stimulated PBMC cultures and mouse models of aging, obesity, and inflammation.

High Precision and Accuracy: Intra-assay CV <10%, inter-assay CV <15%, and accuracy between 82–102% across analytes.

Reveal Hidden Immune Signals: Ideal for detecting low-level cytokine expression that standard assays may miss—critical for understanding early or chronic immune responses.

Other Notes

Sensitivity: Please see protocol for sensitivity of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sha-Sha Yi et al.
Frontiers in psychiatry, 13, 864481-864481 (2022-05-17)
Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
Yingxin Sun et al.
Frontiers in cellular and infection microbiology, 11, 618265-618265 (2021-04-06)
Aging induced chronic systemic inflammatory response is an important risk factor for atherosclerosis (AS) development; however, the detailed mechanism is yet to be elucidated. To explore the underlying mechanism of how aging aggravates AS advancement. A young (five-week-old, YM) and
Hikaru Ito et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 46(2), 442-454 (2020-09-18)
The lateral habenula (LHb) attracts a growing interest as a regulator of monoaminergic activity which were frequently reported to be defective in depression. Here we found that chronic social defeat stress (CSDS) increased production of pro-inflammatory cytokines in LHb associated
Amanda J Martinot et al.
PLoS pathogens, 16(12), e1009096-e1009096 (2020-12-15)
Bacille Calmette-Guerin (BCG), an attenuated whole cell vaccine based on Mycobacterium bovis, is the only licensed vaccine against Mycobacterium tuberculosis (Mtb), but its efficacy is suboptimal and it fails to protect against pulmonary tuberculosis. We previously reported that Mtb lacking
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Related Content

Evaluation of MILLIPLEX® Mouse High Sensitivity T Cell Panel

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

Quality Control Range Sheet- HSTC-108 and HSTC-208
Protocol- MHSTCMAG-70K|MHSTCMAG-70KPMX|MHSTCMAG-70KPXBK
Quality Control Ranges: MILLIPLEX® MAP Mouse High Sensitivity T Cell Magnetic Bead Panel

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