SCC300
3dGRO® Human iPSC Derived Colon Organoids
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About This Item
form
liquid
technique(s)
cell culture | stem cell: suitable
application(s)
cell analysis
General description
3dGRO® Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO® human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO® Human Colon Organoid Expansion Medium (Cat. No. SCM304).
Application
Research Category:
- Cell Culture
- Stem Cell Research
Preparation Note
Analysis Note
- Each vial contains ≥1500 organoids.
- Mycoplasma: Negative
- Genotyped by STR analysis: Pass
- Human species verified
- Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
- Cell viability test: Pass
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
SCC300:
Certificates of Analysis (COA)
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Articles
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.
ヒト上皮性結腸オルガノイドは、Caco-2細胞の薬物透過性アッセイの代わりに、薬物スクリーニングおよび化合物毒性試験に使用できます。
Protocols
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
優れた特性を有する凍結保存状態のヒトiPS細胞由来腸オルガノイドと、iPS細胞から上皮性腸オルガノイドへ分化させるための段階的なオルガノイド培養のプロトコル。
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Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC300 | 04054839989728 |
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