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Single Molecule Counting (SMC™) Erenna® Instrument, Software and Kits

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Singulex Erenna® Immunoassay Instrument
Singulex® Erenna® Instrument with SMC™ technology quantifies low-abundance biomarkers, on the bench top.
The Erenna® Immunoassay system, originally developed by Singulex®, Inc., accurately, precisely and reliably quantifies low-abundance biomarkers at femtogram/mL concentrations. Ultrasensitive Single Molecule Counting (SMC™) technology enables better science by providing better sensitivity.

Scientists use the Erenna® Immunoassay System when they need:
  • Quantification of analyte concentration differences, especially at ultrasensitive levels.
  • Data for biomarkers that were previously undetectable or unquantifiable with traditional immunoassay formats.
  • Unparalleled precision and accuracy, with lower sample volumes and lower analyte concentrations.
  • A method to rapidly develop their own assays with their proprietary antibodies and targets.
  • An enthusiastic partner who will work with them to build assays to suit their discovery needs.
The Erenna® Immunoassay System includes fully integrated benchtop analytical instrumentation with dedicated software for automated data analysis.

MetricSpecification
Slope >20 DE/fM*
Background < 100 DE
LoD < 1 fM*
Precision < 7% CV†
Dynamic Range > 4 logs

*Determined from calibrator set: 0-300 fM of 150 kD antibody labeled with fluorophore †DE measurements from 30 fM calibrator, n=20

Network/PC Requirement

  • Microsoft Windows® 7 Operating Systems
  • A static IP address and an FTP server
  • Sgx link™ software included

Plate Format (Reading)

  • 384-well plate

Instrument Dimensions and Weight

  • H: 400 mm (15.75 in)
  • W: 540 mm (21.25 in)
  • D: 575.6 mm (22.7 in)
  • W: 31.3 kg (69 lbs)

Power Requirement

  • U.S.: 115 VAC, 50-60 Hz (operating range 90-125 V)
  • Int.: 230 VAC, 50-60 Hz (operating range 180-250 V)

Sgx link™ Software

Sgx link™ Software: Connecting Users with Data

  • All Erenna® Immunoassay Systems include proprietary software with a wizard-driven interface for simple operation and analysis of ultrasensitive Erenna® immunoassays. Sgx link™ software allows the user to be in control.
  • Achieve a 4-log+ linear dynamic range with the SMDCurveFit™ algorithm. This proprietary algorithm combines digital and analog data processing for analysis of baseline and elevated samples in the same run.

Wizard-Driven Interface

Step-by-step guide helps operators complete experiment setup and analysis.

Click-and-drag plate layout

Click-and-Drag Plate Layout

Easy-to-use, graphical interface simplifies experiment setup and reduces manual input errors.

Create, Save, and Share Templates

Reusable templates save time and ensure consistent experimental setup.

Multiple Analysis Options

Different curve-fitting modes and outlier removal functions provide fine-tuning control of data analysis (see screenshot to the right).

Variable Data Acquisition Protocols

Read time may be selected (15s – 60s) to best meet your throughput needs and for fit-for-purpose biomarker measurements.

Data Export Options

Automatic generation of final report and multiple file export options help to organize and present data.

User Account and Data Management

Management tools are provided for you to add security and control account privileges to achieve GLP compliance.

SMC™ Immunoassay Kits & Reagents

Singulex Erenna® Immunoassay Kits
SMC™ Immunoassay Kits combine a familiar immunoassay workflow, with a 96-well plate option, with Single Molecule Counting technology
SMC™ Immunoassay Kits using Single Molecule Counting (SMC™) technology (developed by Singulex, Inc.) enable you to reach the highest levels of sensitivity possible in an immunoassay format. SMC™ kits contain all reagents required to perform assays on the Erenna® Immunoassay System. These assays take advantage of a standard immunoassay workflow and may be configured with a proprietary paramagnetic microparticle solid phase capture for ultrasensitive biomarker quantification, or in a standard plate format when extreme sensitivity is not required.

Assay protocols are similar to existing sandwich ELISA methods with two key differences:
  1. Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
  2. SMC™ Immunoassay System detects analytes using Single Molecule Counting (SMC™) technology.
The workflow similarities to a standard ELISA means that a laboratory can convert to SMC™ technology with relative ease. Studies have been performed to demonstrate the ease of conversion from a traditional ELISA to SMC™ immunoassay and the resultant impacts on assay performance, sample requirements, and cost. Download the application note, “Improved Performance with Conversion from ELISA to an SMC™ Immunoassay”.

SMC™ assays for use on the Erenna®

Catalog NoAnalyteSpeciesSample Type
03-0100-01 Akt1 (Ser473) Human, Mouse, Rat Lysate
03-0099-01 Akt1 (Total) Human, Mouse, Rat Lysate
03-0145-00 Amyloid beta 1-40 Human, Mouse, Rat CSF, Plasma
03-0146-00 Amyloid beta 1-42 Human, Mouse, Rat CSF, Plasma
03-0171-00 BDNF Human EDTA Plasma, Serum, CSF
03-0092-00 cTnI Human, Canine, Cynomolgus Monkey, Rat, Guinea Pig Plasma, Serum
03-0154-00 cTnI Human, Canine, Cynomolgus Monkey, Rat, Guinea Pig Plasma, Serum
03-0047-00 G-CSF Human Plasma
03-0024-03 GLP-1 (active) Human, Mouse, Rat, Canine Plasma
03-0025-06 GLP-1 (total) Human, Mouse, Rat, Canine Plasma
03-0153-00 Glucagon Human, Mouse, Rat Plasma
03-0067-00 GM-CSF Human Plasma
03-0049-00 IFN-γ Human Plasma
03-0072-00 IL-1α Human Plasma
03-0167-00 IL-1α Human Plasma, Serum
03-0028-00 IL-1β Human Plasma
03-0160-00 IL-1β Human Plasma, Serum
03-0051-00 IL-2 Human Plasma, Serum
03-0052-00 IL-4 Human Plasma
03-0161-00 IL-4 Human Plasma, Serum
03-0053-00 IL-5 Human Plasma
03-0168-00 IL-5 Human Plasma, Serum
03-0089-01 IL-6 Human Plasma, Serum
03-0155-00 IL-6 Human Plasma, Serum
03-0094-00 IL-7 Human Plasma, Serum
03-0055-00 IL-8 / CXCL8 Human Plasma, Serum
03-0056-00 IL-10 Human Plasma
03-0176-00 IL-10 Human Plasma, Serum
03-0057-00 IL-12 Human Plasma, Serum
03-0156-00 IL-13 Human Plasma, Serum
03-0109-02 IL-13 v2 Human Plasma, Serum
03-0058-00 IL-15 Human Plasma, Serum
03-0159-00 IL-17A Human Plasma, Serum
03-0103-00 IL-17A v2 Human Plasma, Serum
03-0164-00 IL-17F Human Plasma, Serum
03-0102-00 IL-17F v2 Human Plasma, Serum
03-0177-00 IL-17A/F Human Plasma, Serum
03-0119-00 IL-17A/F Heterodimer v2 Human Plasma, Serum
03-0014-07 IL-21 Human Serum
03-0059-01 IL-22 Human Plasma
03-0162-00 IL-22 Human Plasma, Serum
03-0112-00 IL-23 Human Plasma,Serum
03-0157-00 IL-23 Human Plasma, Serum
03-0118-00 KIM-1 Human Plasma, Serum, Urine
03-0163-00 TNFα Human Plasma, Serum
03-0108-00 TNF-α (Mouse) Mouse Serum
03-0088-00 TNF-α (Human) Human Plasma
03-0180-00 VEGF-A Human Plasma, Serum
03-0068-00 VEGF (Human) Human Plasma
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