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Merck

HADK2MAG-61K

MILLIPLEX® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of eight protein biomarkers in human serum and plasma samples.

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

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Product Name

MILLIPLEX® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay, The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of eight protein biomarkers in human serum and plasma samples.

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 87%
(IL-8)
accuracy: 91%
(TNFα)
accuracy: 94%
(Insulin)
standard curve range: 0.64-10,000 pg/mL
(IL-8)
standard curve range: 0.64-10,000 pg/mL
(NGF)
standard curve range: 0.64-10,000 pg/mL
(TNF-α)
standard curve range: 0.96-15,000 pg/mL
(IL-6)
standard curve range: 1.3-20,000 pg/mL
(IL-1β)
standard curve range: 1.3-20,000 pg/mL
(MCP-1)
standard curve range: 38-600,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(HGF)
standard curve range: 9.6-150,000 pg/mL
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-1β)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)

technique(s)

multiplexing: suitable

Quality Level

200

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

Related Categories

Application

  • Analytes: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Download the HADK2MAG-61K xPonentProtocol Template Version 3.1

Biochem/physiol Actions

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.



The MILLIPLEX® Human Adipokine Panel 2 is an 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 samples in duplicate.



Panel Type: Metabolism

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual sensitives.

Packaging

96-well plate
Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Luminex is a registered trademark of Luminex Corp
xMAP is a registered trademark of Luminex Corp

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Waist-to-height ratio, waist circumference and BMI as indicators of percentage fat mass and cardiometabolic risk factors in children aged 3-7 years.
Sijtsma, A; Bocca, G; L'abee, C; Liem, ET; Sauer, PJ; Corpeleijn, E
Clinical Nutrition (Edinburgh, Scotland) null
Insulin resistance is associated with Fibroblast Growth Factor-23 in stage 3-5 chronic kidney disease patients.
Garland, JS; Holden, RM; Ross, R; Adams, MA; Nolan, RL; Hopman, WM; Morton, AR
Journal of Diabetes and Its Complications null
Altered promoter methylation of PDK4, IL1 B, IL6, and TNF after Roux-en Y gastric bypass.
Kirchner, H; Nylen, C; Laber, S; Barres, R; Yan, J; Krook, A; Zierath, JR; Naslund, E
Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery null
Yuek Ling Chai et al.
Scientific reports, 11(1), 4010-4010 (2021-02-19)
Cerebrovascular disease (CeVD) and neurodegenerative dementia such as Alzheimer's disease (AD) are frequently associated comorbidities in the elderly, sharing common risk factors and pathophysiological mechanisms including neuroinflammation. Osteopontin (OPN) is an inflammatory marker found upregulated in vascular diseases as well
Maria A Ramos-Roman et al.
Diabetes, 69(8), 1624-1635 (2020-05-10)
Milk production may involve a transient development of insulin resistance in nonmammary tissues to support redistribution of maternal macronutrients to match the requirements of the lactating mammary gland. In the current study, adipose and liver metabolic responses were measured in
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Global Trade Item Number

SKUGTIN
HADK2MAG-61K-0704053252795930
HADK2MAG-61K-0104053252795879
HADK2MAG-61K-0304053252795893
HADK2MAG-61K-0404053252795909
HADK2MAG-61K-0504053252795916
HADK2MAG-61K-0604053252795923
HADK2MAG-61K-0804053252795947
HADK2MAG-61K-0204053252795886

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