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IPFL07810

Immobilon®-FL PVDF Membrane

1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Product Name

Immobilon®-FL PVDF Transfer Membrane, 0.45 μm pore size, 7 X 8.4 cm sheets

Quality Segment

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and is recommended for blotting proteins with molecular weights greater than 20 kilodaltons (kDa). The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection.
Filter Code: IPFL

Application

Immobilon®-FL Transfer Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Transfer Membrane:
  • Designed for optimal fluorescent immunodetection application
  • Extremely low background improves the sensitivity of all fluorescence based immunodetection
  • Compatible with all commonly used fluorescent probes across a wide range of excitation/emission wavelengths
  • For standard chemiluminescent or chromogenic detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Ryan C Bates et al.
Pharmacology, biochemistry, and behavior, 103(2), 237-244 (2012-09-11)
Valproic acid (VPA) is the most widely prescribed antiepileptic drug due to its ability to treat a broad spectrum of seizure types. However, potential complications of this drug include anticonvulsant polytherapy metabolism, organ toxicity and teratogenicity which limit its use