MAB377A5
Anti-NeuN Antibody, clone A60, Alexa Fluor™ 555 Conjugate
clone A60, from mouse, ALEXA FLUOR™ 555
Synonym(s):
NEUronal Nuclei, Neuronal Nuclei
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 555
Clone:
A60, monoclonal
Application:
ICC, IHC
Citations:
18
biological source
mouse
conjugate
ALEXA FLUOR™ 555
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
A60, monoclonal
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
General description
NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
The unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa
Application
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse adult brain cryosections.
This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN.
Analysis Note
Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.
Other Notes
Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Ferret, Chicken, and Avian.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Madeleine Reilly et al.
Scientific reports, 12(1), 9493-9493 (2022-06-11)
There is an urgent need to develop disease-modifying therapies to treat neurodegenerative diseases which pose increasing challenges to global healthcare systems. Prion diseases, although rare, provide a paradigm to study neurodegenerative dementias as similar disease mechanisms involving propagation and spread
Ángeles Montilla-García et al.
Neuropharmacology, 125, 231-242 (2017-08-02)
Grip strength deficit is a measure of pain-induced functional disability in rheumatic disease. We tested whether this parameter and tactile allodynia, the standard pain measure in preclinical studies, show parallels in their response to analgesics and basic mechanisms. Mice with
Corey G Duke et al.
eNeuro, 7(1) (2019-12-28)
Blue wavelength light is used as an optical actuator in numerous optogenetic technologies employed in neuronal systems. However, the potential side effects of blue light in neurons has not been thoroughly explored, and recent reports suggest that neuronal exposure to
Anna-Kate Fowler et al.
The Journal of biological chemistry, 287(52), 43533-43542 (2012-11-03)
The brain is one of the major targets of chronic alcohol abuse. Yet the fundamental mechanisms underlying alcohol-mediated brain damage remain unclear. The products of alcohol metabolism cause DNA damage, which in conditions of DNA repair dysfunction leads to genomic
Jaycie L Loewen et al.
Journal of neuropathology and experimental neurology, 75(4), 366-378 (2016-03-06)
It is estimated that 30%-40% of epilepsy patients are refractory to therapy and animal models are useful for the identification of more efficacious therapeutic agents. Various well-characterized syndrome-specific models are needed to assess their relevance to human seizure disorders and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB377A5 | 04053252695070 |
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