16669
Magenta-Gal
reagent for selection of recombinant bacterial clones
Synonym(s):
5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
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About This Item
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
PubChem Substance ID:
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
1552602
MDL number:
grade
Molecular Biology
Quality Segment
sterility
non-sterile
assay
≥98.0% (HPLC)
form
powder
solubility
DMF: soluble, DMSO: soluble
suitability
suitable for β-galactosidase test
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
InChI key
CHRVKCMQIZYLNM-RKQHYHRCSA-N
General description
Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.
Biochem/physiol Actions
When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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