H3506
Hyaluronidase from bovine testes
Type I-S, lyophilized powder, 400-1000 units/mg solid
Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
400-1000 units/mg solid
type
Type I-S
form
lyophilized powder
specific activity
400-1000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol Actions
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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