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Merck

M4530

Medium 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Product Name

Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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General description

Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • as a suspension media to maintain the primary neuronal cultures
  • as a component of the collagen gel for ex vivo embryonic heart culture
  • to maintain the ovarian tissue sections as part of the slow-freezing protocol

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

64-17-5

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0091
RNBL9409
RNBL4695
RNBL1897
RNBL1794

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