Skip to Content
Merck

N4645

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
UNSPSC Code:
12352204
NACRES:
NA.75
PubChem Substance ID:
24897708
EC Number:
224-246-5
Beilstein/REAXYS Number:
5324661
MDL number:
MFCD00066288

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

biological source

synthetic (organic)

product line

BioReagent

assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 100 mg/mL

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
  • to treat adherent cells in adhesion assay
  • as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCJ8713
SLCG7475
SLCC9851
SLBW9163
SLBV8429

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections
Chart H, et al.
Journal of Medical Microbiology, 56(9), 1161-1166 (2007)
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray
List C, et al.
PLoS Neglected Tropical Diseases, 4(8), e771-e771 (2010)
Cucurbitacin I inhibits STAT3, but enhances STAT1 signaling in human cancer cells in vitro through disrupting actin filaments
Guo H, et al.
Acta Pharmacologica Sinica, 39(3), 425-425 (2018)
Flexible, actin-based ridges colocalise with the beta1 integrin on the surface of melanoma cells
Poole K and Muller D
British Journal of Cancer, 92(8), 1499-1499 (2005)
Assays for protein-tyrosine phosphatases.
McCain DF and Zhang ZY
Methods in Enzymology, 345, 507-518 (2002)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service