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P4707

Poly-ʟ-Lysine Hydrobromide

Name: Poly-ʟ-Lysine Hydrobromide
Brand: SIGMA

synthetic, liquid, 0.01%, suitable for cell culture

Synonym(s):

PLL Solution

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About This Item

CAS Number:

25988-63-0

UNSPSC Code:

12352202

eCl@ss:

32160406

NACRES:

NA.75

MDL number:

MFCD00165424

Biological source:

synthetic

Form:

liquid

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.01%

Mol wt:

70—150 kDa

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Properties

Product Name

Poly-L-lysine solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

biological source

synthetic

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

form

liquid

mol wt

70—150 kDa

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm²

impurities

endotoxin, tested

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

Description

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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