S1797
SOC Medium
For use in transformation
Synonym(s):
Super Optimal broth
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
sterility
0.2 μm filtered
form
liquid
composition
Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
agriculture
storage temp.
2-8°C
suitability
nonselective for Escherichia coli, nonselective for coliforms
Quality Level
General description
Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
Application
SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.
Features and Benefits
SOC liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package sizes
- Standard formulation
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Meg L Flanagan et al.
Methods in molecular biology (Clifton, N.J.), 378, 33-52 (2008-07-09)
As a source of recombinant antigen, soluble constant fragment (Fc) fusion proteins have become valuable reagents for immunotherapy and laboratory investigations. Additional applications for these reagents include flow cytometry, immunohistochemistry, and in vitro activity assays. To aid investigators in the
Kenneth Smith et al.
Nature protocols, 4(3), 372-384 (2009-02-28)
We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of
Identification of Pathogenicity-Associated Loci in Klebsiella pneumoniae from Hospitalized Patients.
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service