S5761
Sodium bicarbonate
powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture
Synonym(s):
Sodium hydrogen carbonate, soda bicarbonate
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About This Item
Linear Formula:
NaHCO3
CAS Number:
Molecular Weight:
84.01
UNSPSC Code:
12161702
NACRES:
NA.75
PubChem Substance ID:
EC Number:
205-633-8
Beilstein/REAXYS Number:
4153970
MDL number:
Assay:
99.5-100.5%
Grade:
Molecular Biology
Form:
powder
Solubility:
water: soluble 100 mg/mL
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
99.5-100.5%
form
powder
quality
300
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
pH
6.8-7.4
pKa (25 °C)
(1) 6.37, (2) 10.25 (carbonic acid)
solubility
water: soluble 100 mg/mL
density
2.16 g/mL at 25 °C (lit.)
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
General description
Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.
Application
Sodium bicarbonate has been used:
- as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
- as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
- as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
- as a component of medium used for cell culture of human embryonic kidney cells
- as a component of Beltsville thawing solution used for the dilution of semen
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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