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Merck

T8665

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate

form

liquid

storage condition

protect from light

shipped in

wet ice

storage temp.

2-8°C

Quality Level

300

Related Categories

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Disclaimer

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Ready-to-use.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

target_organs

Eyes,Central nervous system

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

207738-08-7 + 67-56-1

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ8626
SLCH1771
SLCD0040
SLCC5061
SLCB5343

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