Water for Pyrogen-Free
Impact of Water
Preparing Pyrogen-free Water using Ultrafiltration
Ultrafiltration is a membrane-based technology that removes endotoxins by a filtration process. Ultrafilters are depth filters with nominal weight cut-off ranging from 5 000 Da to 100 000 Da. To remove endotoxins, filters with a molecular weight from 5 KDa to 13 KDa are selected.Ultrafilters are positioned at the point-of-use, at the outlet of the water purification process. The water delivered is pyrogen-free and does not require autoclaving.
Data shown on Figure 2 demonstrate the efficiency of endotoxin removal by a 13 KDa ultrafilter (BioPak®). This ultrafiltration device is fully efficient over a four month period and large quantities of pyrogens.
Demonstration of endotoxin removal by a 13 KDa ultrafilter (BioPak®)
Conclusions
Ultrafiltration is an efficient and simple way to obtain pyrogen-free water on-demand. It avoids autoclaving procedures on water. Pyrogen-water purified using ultrafiltration can be utilized safely for cell culture, including stem cell culture.The BioPak® ultrafilter comes with a certificate guaranteeing endotoxin-free water for four months when a BioPak point-of-use filter is connected to a high purity water purification system, and utilized in normal laboratory conditions.
More Information
- R&D Notebook – Qualification study of a point-of-use ultrafiltration device for the production of pyrogen-free ultrapure water. S Mabic, D Darbouret, and I Kano.
- Application Note - Endotoxin-free Water for Tissue Culture. E Riché, J Bôle and S Mabic.
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