1.25252
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 100 mm x 25 mm Prep Column
Sinónimos:
Chromolith® Prep RP-18 endcapped 100-25 column
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Nombre del producto
Chromolith® RP-18 desactivada, L × I.D. 100 mm x 25 mm Prep column
material
PEEK column
Quality Segment
agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
1 ea of
extent of labeling
18% Carbon loading
parameter
100 bar pressure, 45 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable (Prep LC), UHPLC-MS: suitable, UHPLC: suitable
column L × I.D.
10 cm × 2 mm
matrix
monolithic silica
matrix active group
C18 (RP18), C18 bonding phase
pore size
12 nm mesoporosity, 3 μm macroporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
General description
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Application
- Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith® RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et al., 2020).
- Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith® RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et al., 2017).
- Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith® RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).
Analysis Note
Theoretical Plates (N/m) (Anthracene): ≥ 40000
Capacity factor (Anthracene): 3.0 - 4.5
Tailing (USP): 0.8 - 1.4 %
separation factor (anthracene/progesterone): 1.9 - 2.5
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