T1895
Thymidine
powder, BioReagent, suitable for cell culture
Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
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About This Item
Empirical Formula (Hill Notation):
C10H14N2O5
CAS Number:
Molecular Weight:
242.23
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-070-4
Beilstein/REAXYS Number:
89285
MDL number:
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
powder
Solubility:
water: 50 mg/mL, clear, colorless
biological source
synthetic (organic)
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
186-188 °C (lit.)
solubility
water: 50 mg/mL, clear, colorless
SMILES string
CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
InChI
1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
InChI key
IQFYYKKMVGJFEH-XLPZGREQSA-N
Gene Information
human ... DTYMK(1841)
General description
Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.
Application
Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
- as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
- as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
- to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells
Biochem/physiol Actions
Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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