233155
Cleland′s Reagent
A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.
Sinónimos:
Cleland’s Reagent, DTT, Dithiothreitol, Dithiothreitol, DTT
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Fórmula empírica (notación de Hill):
C4H10O2S2
Número CAS:
Peso molecular:
154.25
UNSPSC Code:
12161504
NACRES:
NA.25
MDL number:
Assay:
≥97% (titration)
Form:
powder
Servicio técnico
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description
Merck USA index - 14, 3376
assay
≥97% (titration)
form
powder
reaction suitability
reagent type: reductant
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white to off-white
solubility
water: 30 mg/mL, ethanol: soluble
cation traces
heavy metals: ≤1 ppm, heavy metals: <1 ppm
shipped in
ambient
storage temp.
2-8°C
SMILES string
SC[C@H](O)[C@@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChI key
VHJLVAABSRFDPM-IMJSIDKUSA-N
General description
A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
Oxidized dithiothreitol: ≤0.5%.
Application
- Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.: Discusses the role of Cleland′s Reagent in differentiating forms of cyclic nucleotide phosphodiesterase, showcasing its utility in enzymatic studies and its broader implications in biochemistry research (Chassy BM, 1972).
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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