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Merck

516726

Millipore

Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

Synonym(s):

Organic Solvent Mix for DNA/RNA

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.21
Grade:
Molecular Biology
Assay:
≥99% phenol basis (titration)
>99% chloroform basis (HPLC)

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grade

Molecular Biology

Quality Level

100

Assay

≥99% phenol basis (titration)
>99% chloroform basis (HPLC)

form

liquid (Two-layer)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

colorless

shipped in

ambient

storage temp.

2-8°C

General description

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.

Preparation Note

Following equilibration, refrigerate (4°C).

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic (F)

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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