13767
Silica gel orange
with moisture indicator free of heavy metals
Synonym(s):
Silica gel
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About This Item
CAS Number:
UNSPSC Code:
12352309
PubChem Substance ID:
EC Number:
231-545-4
MDL number:
NACRES:
NA.25
quality
with moisture indicator free of heavy metals
Quality Level
impurities
≤0.025% soluble in chloroform
loss
≤2% loss on drying, 150 °C, 3 h
particle size
2-5 mm
capacity
≥25 % absorption capacity (water)
bp
2230 °C
mp
>1600 °C
SMILES string
O=[Si]=O
InChI
1S/O2Si/c1-3-2
InChI key
VYPSYNLAJGMNEJ-UHFFFAOYSA-N
General description
Silica gel orange (Drying pearls Orange) is a commonly employed drying agent.
Application
Silica gel orange (Drying pearls Orange) may be used to provide long-term storage conditions in the following studies:
- Multiwell plates in a gel drying box during the solid-phase reverse transfection on cell microarrays.
- Robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays.
Storage Class
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Holger Erfle et al.
Journal of biomolecular screening, 13(7), 575-580 (2008-07-05)
Solid-phase reverse transfection on cell microarrays is a high-throughput method for the parallel transfection of mammalian cells. However, the cells transfected in this way have been restricted so far to microscopy-based analyses. Analysis methods such as reverse transcriptase-polymerase chain reaction
Holger Erfle et al.
Methods in molecular biology (Clifton, N.J.), 785, 277-287 (2011-09-09)
Endocytosis is one of the most essential cellular processes, which enables cells to internalise diverse -material. It is crucial for regulation of receptor activity and signalling, cell polarisation, attachment and motility, and a great number of other cellular functions. A
Holger Erfle et al.
Nature protocols, 2(2), 392-399 (2007-04-05)
Here, we describe a robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells. The automatic