Skip to Content
Merck

Key Documents

View All Documentation

30970

Sodium deoxycholate

BioXtra, ≥98.0% (dry matter, NT)

Synonym(s):

3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Desoxycholic acid sodium salt

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C24H39NaO4
CAS Number:
302-95-4
Molecular Weight:
414.55
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
57648497
EC Number:
206-132-7
Beilstein/REAXYS Number:
3581950
MDL number:
MFCD00064139
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

ox bile

Quality Level

300

description

anionic

product line

BioXtra

assay

≥98.0% (dry matter, NT)

form

powder

optical activity

[α]20/D +44±2°, c = 2% in H2O

mol wt

micellar avg mol wt 1200-5000

aggregation number

3-12

technique(s)

DNA purification: suitable, RNA purification: suitable, electrophoresis: suitable

impurities

≤1% cholic acid (HPLC), ≤6% water

CMC

2-6 mM (20-25°C)

solubility

water: soluble 1 gm in 10 ml, clear, colorless to very faintly yellow

cation traces

Ca: ≤500 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤10 mg/kg, Mg: ≤10 mg/kg, Mn: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤10 mg/kg, Zn: ≤5 mg/kg

HLB

16

functional group

carboxylic acid

SMILES string

[Na+].[H][C@]12CC[C@@]3([H])[C@]4([H])CC[C@H]([C@H](C)CCC([O-])=O)[C@@]4(C)[C@@H](O)C[C@]3([H])[C@@]1(C)CC[C@@H](O)C2

InChI

1S/C24H40O4.Na/c1-14(4-9-22(27)28)18-7-8-19-17-6-5-15-12-16(25)10-11-23(15,2)20(17)13-21(26)24(18,19)3;/h14-21,25-26H,4-13H2,1-3H3,(H,27,28);/q;+1/p-1/t14-,15-,16-,17+,18-,19+,20+,21+,23+,24-;/m1./s1

InChI key

FHHPUSMSKHSNKW-SMOYURAASA-M

General description

Sodium Deoxycholate, a bile acid derivative, is a crucial component in biochemical and cell biology research. This water-soluble detergent is widely used in protein-related experiments, including cell lysates and liposome preparation. It aids in membrane protein extraction, lipid compound extraction, and cell nucleus separation. Sodium Deoxycholate is also applied in cholesterol flocculation tests and affinity chromatography for column elution or regeneration. It is commonly used in microbiology for cell lysing, especially because it can be removed from the solution via dialysis.

Sodium Deoxycholate is also utilized in eluting buffers for affinity chromatography and plays a crucial role in DNA and RNA extraction from cells and tissues in molecular biology applications. Additionally, it is used as a catalyst in the preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.

Application

Sodium deoxycholate has been used for preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.

Biochem/physiol Actions

Sodium deoxycholate is a bile salt that stimulates the apical to basolateral accumulation of epirubicin in Caco-2 cells as well as increased serosal absorption of epirubicin in the rat jejunum and ileum. Sodium deoxycholate dissociates histones II from DNA as well as causes lysis of the viral membrane.

Features and Benefits

  • Suitable for Cell Biology and Biochemical Research
  • Can be used in Electrophoresis, DNA and RNA purification
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
  • High purity product for research applications

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

Still not finding the right product?

Explore all of our products under Sodium deoxycholate

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Selective dissociation of histones from chromatin by sodium deoxycholate.
J E Smart et al.
Journal of molecular biology, 58(3), 651-659 (1971-06-28)
Y L Lo et al.
Biochemical pharmacology, 59(6), 665-672 (2000-02-25)
The effects of sodium deoxycholate (Deo-Na), a bile salt, and sodium caprate (Cap-Na), a fatty acid, on the transport of epirubicin were investigated in both the human colon adenocarcinoma (Caco-2) cell line and the everted gut sacs of the rat
A Helenius et al.
Biochimica et biophysica acta, 436(2), 319-334 (1976-06-17)
The effects of increasing concentrations of sodium deoxycholate on Semliki Forest have been studied. Sodium deoxycholate begins to bind to the virus at less than 0.1 mM free equilibrium concentration and causes lysis of the viral membrane at 0.9 +/-
View All Related Papers