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Merck

A4284

L-Asparagine monohydrate

BioReagent, suitable for cell culture

Synonym(s):

(S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide

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About This Item

Linear Formula:
NH2COCH2CH(NH2)COOH · H2O
CAS Number:
5794-13-8
Molecular Weight:
150.13
UNSPSC Code:
12352209
NACRES:
NA.75
PubChem Substance ID:
24890843
EC Number:
200-735-9
Beilstein/REAXYS Number:
1723527
MDL number:
MFCD00151038

Key Documents

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biological source

synthetic

Quality Level

300

product line

BioReagent

assay

≥99% (TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

color

white to off-white

mp

233-235 °C (lit.)

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

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Application

L-Asparagine monohydrate has been used:
  • as a media supplement to culture Black Mexican Sweet (BMS) cells
  • to prepare Adams III media to supplement C+Y medium for culturing Streptococcus pneumoniae
  • in an asparagine-rich diet to study the E0771 syngeneic breast tumor growth and survival in mice

Biochem/physiol Actions

L-Asparagine (L-Asp) is a nonessential amino acid involved in the synthesis of critical cellular proteins of human cells. It is synthesized from oxaloacetic acid. L-Asp is used as a growth supplement for tumor cells in in vitro conditions.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
108K1353
127K0760
125K0721
125K07212
125K07211

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Neurologic complications of chemotherapy and other newer and experimental approaches
Soffietti R, et al.
Handbook of Clinical Neurology, 1199-1218 null
Ana Romina Fox et al.
The New phytologist, 228(3), 973-988 (2021-01-08)
Plasma membrane (PM) intrinsic proteins (PIPs) are aquaporins facilitating the diffusion of water and small solutes. The functional importance of the PM organisation of PIPs in the interaction with other cellular structures is not completely understood. We performed a pull-down
Deia Abd El-Hady et al.
Food chemistry, 188, 551-558 (2015-06-05)
Acrylamide (AA) is a known lethal neurotoxin and carcinogen. AA is formed in foods during the browning process by the Maillard reaction of glucose (GL) with asparagine (AS). For the first time, the simultaneous online preconcentration and separation of AA
Gustavo Luis Leonardo Scalone et al.
Journal of agricultural and food chemistry, 63(22), 5364-5372 (2015-05-15)
Pyrazines are specific Maillard reaction compounds known to contribute to the unique aroma of many products. Most studies concerning the generation of pyrazines in the Maillard reaction have focused on amino acids, while little information is available on the impact
Han Yao et al.
Journal of pharmaceutical and biomedical analysis, 117, 232-239 (2015-09-18)
L-asparaginase is an effective anti-tumor agent for acute lymphoblastic leukemia. This work presents the development of an activity determination of L-ASNase preparations for pharmaceutical quality control purposes, in accordance with analytical Quality by Design principles. Critical method attributes, the absorbance

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