D225
4,5-Diaminofluorescein diacetate solution
5 mM in DMSO, ≥97% (HPLC)
Synonym(s):
DAF-2 DA
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About This Item
Quality Level
assay
≥97% (HPLC)
form
solution
availability
not available in Japan
concentration
5 mM in DMSO
shipped in
wet ice
storage temp.
−20°C
General description
4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs). DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.
Application
Cell-permeable fluorescent detector of nitric oxide in living cells.
Legal Information
DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590
US Patent No. 5,874,590
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA
Takeno S, et al.
Clinical and Experimental Allergy, 31(6), 881-888 (2001)
Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
Qiu W, et al.
Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)
Anne Marie W Bartosch et al.
Biophysical journal, 113(1), 101-108 (2017-07-13)
Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells.