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Merck

D5758

Diethyl pyrocarbonate

96% (NT)

Synonym(s):

DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

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About This Item

Linear Formula:
O(COOC2H5)2
CAS Number:
1609-47-8
Molecular Weight:
162.14
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
24893964
EC Number:
216-542-8
Beilstein/REAXYS Number:
637031
MDL number:
MFCD00009106

Key Documents

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InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

SMILES string

CCOC(=O)OC(=O)OCC

biological source

synthetic (microbial fermentation/ cell culture)

assay

96% (NT)

form

liquid

Quality Level

200

refractive index

n20/D 1.398 (lit.)

bp

93-94 °C/18 mmHg (lit.)

density

1.101 g/mL at 25 °C (lit.)

shipped in

wet ice

storage temp.

2-8°C

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General description

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

Application

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Biochem/physiol Actions

DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase.
It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

10 - Combustible liquids

flash_point_f

156.2 °F - closed cup

flash_point_c

69 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF8918
BCCD9704
BCCD9249
BCCD6206
BCCD2241

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H Perry-O'Keefe et al.
Journal of applied microbiology, 90(2), 180-189 (2001-02-13)
A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of
Properties of mouse globin messenger ribonucleic acid and its preparation in milligram quantities.
R Williamson et al.
Biochemistry, 10(16), 3014-3021 (1971-08-03)
V Shoshan-Barmatz et al.
The Biochemical journal, 299 ( Pt 1), 177-181 (1994-04-01)
Exposure of junctional sarcoplasmic reticulum (SR) membranes or purified ryanodine receptor to the histidine-specific reagent diethyl pyrocarbonate (DEPC) led to concentration- and time-dependent inactivation of ryanodine binding. The pH-dependence of the inactivation of ryanodine binding by DEPC and the reversal
E C Williamson et al.
Journal of clinical microbiology, 39(1), 47-50 (2001-01-04)
Respiratory tract colonization with Scedosporium apiospermum in patients with chronic suppurative lung disease is a significant concern for lung transplantation candidates, since Scedosporium infections occurring posttransplantation are usually untreatable. Up to 10% of patients with cystic fibrosis attending our respiratory
A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues.
F Solymosy et al.
European journal of biochemistry, 5(4), 520-527 (1968-09-24)
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