F2426
EZview™ Red ANTI-FLAG® M2 Affinity Gel
clone M2
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Application:
IP, affinity chromatography
Citations:
98
clone
M2, monoclonal
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
4% agarose bead; 45-165μm bead size
isotype
IgG1
capacity
≥0.6 mg/mL, gel binding capacity
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.
Application
Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.
Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide
Learn more product details in our FLAG® application portal.
Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG® Affinity Gels, FLAG® tag, 3xFLAG®tag, DYKDDDDK tag
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
Matthew Frieman et al.
Journal of virology, 83(13), 6689-6705 (2009-04-17)
The outcome of a viral infection is regulated in part by the complex coordination of viral and host interactions that compete for the control and optimization of virus replication. Severe acute respiratory syndrome coronavirus (SARS-CoV) intimately engages and regulates the
Related Content
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service