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Merck

G0376

β-Glucuronidase from bovine liver

Type B-3, ≥2,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC Number:
Specific activity:
≥2,000,000 units/g solid
Biological source:
bovine liver
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biological source

bovine liver

Quality Level

type

Type B-3

form

solid

specific activity

≥2,000,000 units/g solid

storage temp.

−20°C

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).


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pictograms

Health hazard

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1



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Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
Klotho to treat kidney fibrosis.
Maria D Sanchez-Niño et al.
Journal of the American Society of Nephrology : JASN, 24(5), 687-689 (2013-04-20)
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded