H9914
HIS-Select® Nickel Magnetic Agarose Beads
Synonym(s):
nickel charged magnetic beaded agarose
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About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
Form:
suspension
conjugate
magnetic beads
Quality Level
form
suspension
shelf life
2 yr (Unopened product)
matrix
6% beaded magnetic agarose
capacity
≥15 mg/mL binding capacity
storage temp.
2-8°C
General description
HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.
Application
The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.
Physical form
Supplied as a 50% slurry suspension in 30% Ethanol.
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
86.0 °F
flash_point_c
30 °C
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