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P5493

Phosphate buffered saline

Name: Phosphate buffered saline
Brand: SIGMA

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

UNSPSC Code:

12161700

eCl@ss:

32129211

NACRES:

NA.25

MDL number:

MFCD00131855

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Properties

Product Name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

Quality Level

100, 200

grade

BioPerformance Certified, Molecular Biology

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable, immunocytochemistry: suitable, immunofluorescence: suitable

impurities

Bioburden, tested, endotoxin, tested, ≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for , suitable for flow cytometry, suitable for microscopy

application(s)

advanced drug delivery

foreign activity

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Description

Application

Phosphate buffered saline has been used:

as a cell wash solution in human dental pulp stem cells (DPSCs)
for perfusion studies in dissected brain samples
as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
as wash solution for ovary and sperm during in vitro culture and fertilization

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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