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34115-M

N-Ethylmaleimide

≥98% (HPLC), H+-ATPase inhibitor, solid

Synonym(s):

N-Ethylmaleimide, NEM

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About This Item

Empirical Formula (Hill Notation):
C6H7NO2
CAS Number:
128-53-0
Molecular Weight:
125.13
UNSPSC Code:
12352111
MDL number:
MFCD00005509
NACRES:
NA.77
Assay:
≥98% (HPLC)
Form:
solid
Storage condition:
OK to freeze
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Product Name

N-Ethylmaleimide, Sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells.

description

Merck USA index - 14, 3822

Quality Segment

100

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

ethanol: 5 mg/mL

storage temp.

10-30°C

SMILES string

N1(C(=O)C=CC1=O)CC

InChI

1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3

InChI key

HDFGOPSGAURCEO-UHFFFAOYSA-N

General description

Sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA2 in endothelial cells.
Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei.

Biochem/physiol Actions

Primary Target
H+-ATPase
Target IC50: 22 µM in suppressing short circuit current

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Corrosive (L)

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pictograms

Skull and crossbonesCorrosion
GHS06,GHS05

signalword

Danger

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

163.2 °F - closed cup

flash_point_c

72.9 °C - closed cup

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