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About This Item
UNSPSC Code:
41105600
NACRES:
NA.54
Specific activity:
≥30 U/mg
Biological source:
bovine pancreas
Concentration:
10 mg/mL
biological source
bovine pancreas
form
solution
specific activity
≥30 U/mg
packaging
pkg of 1 mg (10 mg/ml)
manufacturer/tradename
Roche
concentration
10 mg/mL
General description
A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.
Application
High concentration, DNase-free RNase has been used for:
- Genomic DNA isolation
- Isolation of plasmid DNA
- Degradation of RNA for flow cytometry
Preparation Note
Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Analysis Note
Absence of DNase activity tested according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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