85469
SOC Broth
suitable for microbiology
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106204
sterility
sterile (Filtered and Aseptic Handled)
Quality Level
form
powder
composition
glucose, 20 mM , magnesium chloride, 10 mM , magnesium sulfate, 10 mM , potassium chloride, 2.5 mM , sodium chloride, 10 mM , tryptone, 20 g/L , yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
final pH
7.0±0.2 (25 °C)
application(s)
food and beverages
life science and biopharma, microbiology
storage temp.
2-8°C
suitability
nonselective for Escherichia coli, nonselective for coliforms
Application
SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.
Preparation Note
Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Transformation of E. coli with plasmids
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Related Content
Advanced Bacterial Genetics, Cold Spring Harbor (2007)
F.M. Ausubel et al.
Current Protocols in Molecular Biology (1987)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth