C6745
Human Collagen Type IV
from human cell culture, liquid, 0.3 mg/mL, suitable for cell culture
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About This Item
Biological source:
human cell culture
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.3 mg/mL
Mol wt:
170—180 kDa
Product Name
Collagen Type IV from human cell culture, Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
biological source
human cell culture
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
170—180 kDa
packaging
pkg of 1 mL
concentration
0.3 mg/mL
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
impurities
HBSAG, none detected, HCV, none detected, HIV-1/HIV-2, none detected
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Laminin
, Peptide Source: Nidogen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... COL4A1(1282)
General description
COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats . It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .
Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.
Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.
Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.
Application
Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.
Biochem/physiol Actions
COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.
Features and Benefits
- This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
- The Collagen Type IV is then purified biochemically.
- It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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