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제품정보 (DICE 배송 시 비용 별도)
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human breast (adenocarcinoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid to hypotetraploid
Morphology:
Epithelial-like
biological source
human breast (adenocarcinoma)
packaging
tube of 5 μg 86012803-DNA-5UG, pkg of vial of cells 86012803-1VL
growth mode
Adherent
karyotype
2n = 46, hypertriploid to hypotetraploid
morphology
Epithelial-like
products
Not specified
receptors
Reported to express the oestrogen and progesterone receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Type B and C viruses
Biochem/physiol Actions
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Human Caucasian breast adenocarcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
Other Notes
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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