F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
16.0 kDa
Impurities:
≤1.00 EU/μg
Product Name
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
Quality Segment
biological source
human
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF2 (2247)
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Application
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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