G5635
β-Galactosidase from Escherichia coli
Grade VIII, lyophilized powder, ≥500 units/mg protein
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
≥500 units/mg protein
type
Grade VIII
Quality Segment
form
lyophilized powder
specific activity
≥500 units/mg protein
mol wt
465 kDa
does not contain
BSA as extender
composition
Protein, ≥60% biuret
storage temp.
−20°C
General description
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. It may be used as a positive control protein with anti-β-galactosidase antibodies.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical form
Contains Tris buffer salts and magnesium chloride
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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