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N7885

Neuraminidase from Vibrio cholerae

Name: Neuraminidase from <I>Vibrio cholerae</I>
Brand: SIGMA

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

Synonym(s):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

CAS Number:

9001-67-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-624-6

MDL number:

MFCD00131711

EC Number:

3.2.1.18 (BRENDA, IUBMB)

Specific activity:

1-5 units/mg protein (Lowry, using NAN-lactose)

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Properties

Quality Level

300

type

Type III

form

buffered aqueous solution

specific activity

1-5 units/mg protein (Lowry, using NAN-lactose)

mol wt

83 kDa

application(s)

diagnostic assay manufacturing

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

Description

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Biochem/physiol Actions

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Preparation Note

Chromatographically purified.

Analysis Note

Preservative free.

Other Notes

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.