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Merck

P5493

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:
MFCD00131855

Key Documents

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Product Name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

grade

BioPerformance Certified, Molecular Biology

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

Quality Level

100, 200

technique(s)

cell culture | mammalian: suitable, immunocytochemistry: suitable, immunofluorescence: suitable

impurities

Bioburden, tested, endotoxin, tested, ≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for , suitable for flow cytometry, suitable for microscopy

application(s)

advanced drug delivery

foreign activity

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

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Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)
  • for perfusion studies in dissected brain samples
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
  • as wash solution for ovary and sperm during in vitro culture and fertilization

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL3386
SLCL7075
SLCL8416
SLCL7076
SLCL4813

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Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)
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