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P8203

PIPES

Name: PIPES
Brand: SIGMA

BioXtra, ≥99% (titration)

別名:

1,4-ピペラジンジエタンスルホン酸, ピペラジン-1,4-ビス(2-エタンスルホン酸), ピペラジン-N,N′-ビス(2-エタンスルホン酸)

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この商品について

実験式（ヒル表記法）:

C₈H₁₈N₂O₆S₂

CAS番号:

5625-37-6

分子量:

302.37

UNSPSC Code:

12161700

NACRES:

NA.25

PubChem Substance ID:

24898917

EC Number:

227-057-6

Beilstein/REAXYS Number:

817713

MDL number:

MFCD00006159

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特性

product line

BioXtra

Quality Segment

400

assay

≥99% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.5% (as SO₄)

loss

≤0.5% loss on drying, 110°C

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤0.2%

cation traces

Al: ≤0.005%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.005%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.1%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.1 at 260 in 1 M NaOH at 0.5 M, ≤0.1 at 280 in 1 M NaOH at 0.5 M

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

詳細

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.^,
PIPES has been utilized in protein crystallization.^, The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Analysis Note

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

Other Notes

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

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保管分類

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c