R7647
Random Nonamers
for use as primers in cDNA synthesis, 50 μM in H2O
Synonym(s):
Oligodeoxynucleotides
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About This Item
NACRES:
NA.31
UNSPSC Code:
41105500
Form:
liquid
Storage temp.:
−20°C
grade
Molecular Biology
Quality Level
form
liquid
usage
0.1 mL sufficient for 100 RT-PCR reactions
concentration
50 μM in H2O
shipped in
wet ice
storage temp.
−20°C
General description
Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.
Application
Random Nonamers are suitable for:
- First-strand cDNA synthesis
- Preparing cDNA libraries (with oligo (dT) primers
- Prepare labeled DNA probes for hybridizations
Features and Benefits
- When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
- In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
- The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
- Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.
Other Notes
For laboratory use only. Not for drug, household, or other uses.
Random Nonamers are provided as a 50 uM solution in water.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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