S7901
Stem Cell Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable
동의어(들):
SCF, c-Kit ligand
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제품정보 (DICE 배송 시 비용 별도)
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
monomer 18.6 kDa ((165 aa))
Impurities:
≤1 EU/μgtested (LAL test)
제품 이름
Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
powder
potency
≤15 ng/mL ED50
quality
endotoxin tested
mol wt
monomer 18.6 kDa ((165 aa))
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
Application
Stem Cell Factor human has been used in
- In vitro differentiation of equine embryonic stem cells
- Chemotaxis detection system
- Ex vivo expansion of umbilical cord blood CD34+ cells
- Cytokine therapy
Biochem/physiol Actions
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.
Physical form
Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Analysis Note
The proliferative activity is tested in culture using TF1 human erythroleukemic cells.
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저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)