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MAB3839 Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3

Detect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC.

Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer, Metabolism Research Sub-Category: Apoptosis - Additional, Enzymes & Biochemistry Gene Symbol: TGM2, TG, TGC, TG2, C, protein-glutamine-gamma-glutamyltransferase, Transglutaminase-2, TGase-H, EC 2.3.2.13 UniProt Number: P21980
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MAB3839
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3839
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3
      Alternate Names
      • tTG
      • Protein-glutamine gamma-Glutamyltransferase
      • Transglutaminase 2
      • TGase C
      Background InformationTissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues (Monsonego, 1997). Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation.

      High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.
      References
      Product Information
      FormatPurified
      PresentationProtein A purified immunoglobulin in 0.02M Phosphate Buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationDetect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunohistochemistry: 20 μg/mL

      Adhesion Blocking: 10-20 μg/mL (per 2x10e5 cells). 4G3 interferes with adhesion of TPA stimulated THP-1 cells and M-CSF stimulated peripheral blood monocytes to fibronectin.

      Optimal working dilutions must be determined by the end user.



      PROTOCOL:

      IMMUNOCYTOCHEMISTRY: THP-1 cells were induced to differentiate into macrophages by treatment with 150 ng/ml of TPA and plated on fibronectin coated glass coverslips for 72 hours.Live non-permeablized cells were incubated with 20 μg/mL of MAB3839 for 45 minutes, washed three times with PBS and fixed for 10 minutes with 3.7% paraformaldehyde. The cells were then washed with PBS and incubated 45 minutes with a fluoresceine labeled affinity-purified goat-anti-mouse secondary antibody. After extensive washing with PBS, cells were mounted in Fluorsave medium.

      Observed Staining:Cell surface tTG was colocalized with b1- and b3-Integrins in podosome-like adhesive structures of macrophages adherent on fibronectin.
      Biological Information
      ImmunogenFibronectin binding domain of tissue transglutaminase (amino acids 1-165 of tTG).
      EpitopeFN binding domain
      Clone4G3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityTissue Transglutaminase. Will recognize all three known splice variants of tTG.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTransglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • TGM2
      • TG
      • TGC
      • TG2
      • C
      • protein-glutamine-gamma-glutamyltransferase
      • Transglutaminase-2
      • TGase-H
      • EC 2.3.2.13
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P21980 # Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
      COFACTOR: Binds 1 calcium ion per subunit (By similarity).
      SIZE: 687 amino acids; 77329 Da
      SUBUNIT: Monomer.
      SIMILARITY: SwissProt: P21980 ## Belongs to the transglutaminase superfamily. Transglutaminase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MAB3839 04053252724985

      Documentation

      Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 Certificates of Analysis

      TitleLot Number
      Mouse Anti-Human Tissue Transglutaminase MOUSE ANTI-HUMAN TIS
      Mouse Anti-Human Tissue Transglutaminase Monoclonal Antibody - 2421571 2421571
      Mouse Anti-Human Tissue Transglutaminase Monoclonal Antibody - 2454730 2454730
      Mouse Anti-Human Tissue Transglutaminase -2562074 2562074
      Mouse Anti-Human Tissue Transglutaminase -2626731 2626731
      Mouse Anti-Human Tissue Transglutaminase -2665081 2665081
      Mouse Anti-Human Tissue Transglutaminase -2673144 2673144
      Mouse Anti-Human Tissue Transglutaminase -2684239 2684239
      Mouse Anti-Human Tissue Transglutaminase -2698538 2698538
      Mouse Anti-Human Tissue Transglutaminase -2703766 2703766
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies