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Notre large gamme est constituée de panels multiplex qui vous permettent de choisir, au sein d'un panel, les analytes qui répondent le mieux à vos besoins. Sur un autre onglet, vous pouvez choisir un format cytokine préconfiguré ou un kit Simplex.
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Choisissez des kits préconfigurés qui permettent d'explorer l'ensemble des voies ou des processus. Ou concevez vos propres kits en choisissant des Simplex MAPmate™ et en suivant les instructions fournies.
Les MAPmate™ suivants ne peuvent pas être utilisés ensemble : -des MAPmate™ qui nécessitent des tampons différents -des paires de MAPmate™ totaux et phospho-spécifiques, par ex. GSK3β total et GSK3β (Ser 9) -des MAPmate™ PanTyr et spécifiques d'un site, par ex. Récepteur Phospho-EGF et phospho-STAT1 (Tyr701) -Plus d'un phospho-MAPmate™ pour une seule cible (Akt, STAT3). -GAPDH et β-Tubuline ne peuvent pas être utilisés avec les kits ou les MAPmate™ contenant panTyr.
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Ajouter des réactifs supplémentaires (Un kit "Buffer and Detection Kit" est nécessaire pour une utilisation avec les MAPmate™)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Option de gain de place Nos clients qui commandent plusieurs kits peuvent choisir d'économiser de l'espace de stockage en éliminant l'emballage de chaque kit et de recevoir les composants de leur essai multiplex conditionnés sous poches en plastique pour un stockage plus compact.
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Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay.
More>>Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay. Less<<
Anti-phospho-FAK (Tyr397) Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
References
Product Information
Format
Affinity Purified
Control
untransfected and Src-transfected RAT2 cell lysates
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq].
FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Molecular Weight
~125 kDa observed. An uncharacterized band appears at ~190 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
