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Merck

123072

Luminol

97%

Synonym(s):

3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

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About This Item

Empirical Formula (Hill Notation):
C8H7N3O2
CAS Number:
521-31-3
Molecular Weight:
177.16
UNSPSC Code:
12352103
NACRES:
NA.23
PubChem Substance ID:
24847519
EC Number:
208-309-4
Beilstein/REAXYS Number:
383929
MDL number:
MFCD00006890

Key Documents

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Product Name

Luminol, 97%

InChI key

HWYHZTIRURJOHG-UHFFFAOYSA-N

InChI

1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)

SMILES string

Nc1cccc2C(=O)NNC(=O)c12

assay

97%

mp

>300 °C (lit.)

Quality Level

200

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Application

  • As a chemiluminiscent substance. Used to measure opsonic and phagocytic function.
  • As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency.
  • Used as a forensic test for blood.
Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminol/peroxidase-based assay.

Features and Benefits

  • Very effective chemiluminiscent compound.
  • Can detect sub-part per billion levels of metal ions with simple and inexpensive instrumentation.
Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.

General description

Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent, light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN9858
SHBN6772
SHBN6769
SHBN1927
SHBN1928

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Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere
Marley, Nancy A., et al.
The Review of Scientific Instruments, 75, 4595-4605 (2004)
T I Quickenden et al.
Luminescence : the journal of biological and chemical luminescence, 16(4), 295-298 (2001-08-21)
A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and commercial
Mechanism of cobalt catalysis of luminol chemiluminescence
Burdo, Timothy G., and W. Rudolf Seitz
Analytical Chemistry, 47, 1639-1643 (1975)
C Dahlgren et al.
Infection and immunity, 39(2), 736-741 (1983-02-01)
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence, linked to activation of the oxidative metabolism of the cells. PMNL isolated from a patient with a myeloperoxidase deficiency were found to produce almost no luminol-dependent
C S Easmon et al.
Immunology, 41(1), 67-74 (1980-09-01)
Luminol-dependent chemiluminescence produced by peripheral blood phagocytic cells was measured using a light detecting instrument, the Luminometer 1250 (LKB Wallac). Although less sensitive than liquid scintillation counters, the Luminometer can measure chemiluminescence effectively and detect experimentally induced and clinical variations
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