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Merck

330000

Rose bengal

Dye content 95 %, ≥96% (HPLC)

Synonym(s):

4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt

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About This Item

Empirical Formula (Hill Notation):
C20H2Cl4I4Na2O5
CAS Number:
632-69-9
Molecular Weight:
1017.64
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24859827
EC Number:
211-183-3
Beilstein/REAXYS Number:
3645857
MDL number:
MFCD00005043

Key Documents

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Quality Level

200

assay

≥96% (HPLC)

form

solid

composition

Dye content, 95%

technique(s)

titration: suitable

color

red to brown

ε (extinction coefficient)

≥ 3000 at 350-354 nm, ≥12000 at 311-315 nm, ≥32000 at 262-266 nm, ≥32000 at 513-517 nm, ≥98000 at 546-550 nm at 0.04 g/L (H2O + 1ml 1% Na2CO3)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24

InChI

1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2

InChI key

UWBXIFCTIZXXLS-UHFFFAOYSA-L

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General description

Rose Bengal is used to stain dead and degenerating cells and mucus.
Rose Bengal is a red or rose color dye and is anionic in nature. It is a 4,5,6,7-tetrachloro 2′,4′,5′,7′-tetraiodo derivative of fluorescein. It is widely used to detect damage to the ocular surface epithelium in ocular surface diseases such as dry eye and herpetic keratitis. Rose Bengal has mild antiviral potency.

Application

Rose Bengal has been used to evaluate acrosome integrity. It has also been used to initiate photothrombotic occlusion in blood.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

330000-VAR: + 330000-BULK: + 330000-1G:4548173987989 + 330000-5G:4548173987996

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Photothrombotic induction of capillary ischemia in the mouse cortex during in vivo two-photon imaging
Underly RG and Shih AY
Bio-protocol, 7(13) (2017)
MicroRNA-181b inhibits thrombin-mediated endothelial activation and arterial thrombosis by targeting caspase recruitment domain family member 10
Lin J, et al.
Faseb Journal, 30(9), 3216-3226 (2016)
Mucin Characteristics of Human Corneal-Limbal Epithelial Cells that Exclude the Rose Bengal Anionic Dye
Argueso P
Investigative Ophthalmology & Visual Science, 47, 113-119 (2006)
Equine Ophthalmology (2011)
New insights for male infertility revealed by alterations in spermatic function and differential testicular expression of thyroid-related genes
Romano RM, et al.
Endocrine, 55(2), 607-617 (2017)
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