Skip to Content
Merck

Key Documents

View All Documentation

341738

Sulforhodamine B, acid form

Dye content 95 %

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C27H30N2O7S2
CAS Number:
2609-88-3
Molecular Weight:
558.67
NACRES:
NA.23
PubChem Substance ID:
57649121
UNSPSC Code:
12352103
EC Number:
220-025-2
MDL number:
MFCD00067690
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solid

Quality Level

200

composition

Dye content, 95%

mp

>300 °C (lit.)

λmax

558 nm

SMILES string

CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)

InChI key

IOOMXAQUNPWDLL-UHFFFAOYSA-N

General description

Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.

Application

pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
11917BU
05825DO
03531JW
01020MG
20223EA

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

バイオテクノロジーにおけるグラフェン

グラフェンベースのナノ材料は、DNA、たんぱく質、細胞、標的薬剤、ポリマー、ナノ粒子などと結合可能であり、多用な化学的性質を有するため、生物医学研究のための魅力的なナノプラットフォームとなります。

蛍光消光顕微鏡法：

蛍光消光顕微鏡は、グラフェンやMoS2のような2次元材料を、迅速かつ安価に、高い忠実度で可視化する。

Graphene in Biotechnology

Graphene is the building block for carbon nanomaterials with different dimensionalities.

View All Articles
Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Minerva Nájera-Martínez et al.
Inhalation toxicology, 24(11), 762-773 (2012-09-08)
Halomethanes (HMs) can be formed during the chlorination process to obtain drinking water. In liver cells, HMs had been shown to be mutagenic and carcinogenic; however, their bioactivation by CYP 2E1 and GSTT1 is required. Although inhalation is the most
View All Related Papers