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04-928

Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

culture supernatant, clone A3S, Upstate®

Synonym(s):

H3, Histone H3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
A3S, monoclonal
Species reactivity:
human, rat, Saccharomyces cerevisiae, mouse, chicken
Application:
ChIP, WB
Citations:
22
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biological source

rabbit

Quality Segment

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

A3S, monoclonal

species reactivity

human, rat, Saccharomyces cerevisiae, mouse, chicken

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... H3F3B(3021)

General description

17 kDa
An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.

Immunogen

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3

Application

Anti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.

Biochem/physiol Actions

Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3.

Physical form

Cultured supernantant in 0.05% sodium azide

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Routinely evaluated by immunoblot analysis.

Other Notes

Replaces: 05-928

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

04-928:

jan

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